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  • MEM(含NEAA)
  • MEM(含NEAA)

MEM(含NEAA)

貨號:PM150410

規格:500mL

價格: ¥58

數量: - +
  • MEM(含NEAA)
  • MEM(含NEAA)
產品概述

MEM培養基(Minimum Essential Medium)也稱最低必需培養基、最小基本培養基或低限量Eagle培養基,由Harry Eagle在Eagle基本培養基(BEM)的基礎上發展而來的,是一種最基本、適用范圍最廣的培養基,是動物細胞培養中最常用的培養基之一。MEM培養基僅含有12種必需氨基酸、谷氨酰胺和8種維生素,成分簡單,主要用于貼壁細胞的培養,配方修改后也可用于其他類型細胞培養。

MEM含NEAA(非必須氨基酸)的培養基,是在MEM培養基的基礎上添加L-丙氨酸、L-谷氨酸、L-天(門)冬酰胺、L-天(門)冬氨酸、L-脯氨酸、L-絲氨酸和甘氨酸7種NEAA,能降低細胞培養時細胞自身生產非必須氨基酸的副作用,有效促進細胞增殖代謝。

本產品含有多類細胞培養所需的氨基酸、維生素、無機鹽等多種成分,但不含蛋白質、脂類或任何生長因子,故此產品需搭配血清或無血清添加物使用。

成分說明

形態

液體

濃度

規格

500mL

PH

7.27.4

平衡鹽

Earle’s

NEAA

L-谷氨酰胺

2mM

NaHCO3

2200mg/L

D-葡萄糖

1000mg/L

HEPES緩沖劑

酚紅指示劑

10mg/L

儲存條件

28℃,避光

運輸條件

常溫

有效期

12個月

注意事項

1、本產品經過濾除菌,使用時應注意無菌操作,避免污染;

2、為保持本產品的最佳使用效果,請勿進行凍融處理;

3、本產品僅用于科研或進一步研究使用,不用于診斷和治療。


參考文獻
文獻列表
  • Blood–brain barrier injury and neuroinflammation induced by SARS-CoV-2 in a lung–brain microphysiological system(2023/06/22)

    作者:Peng Wang, Lin Jin, Min Zhang

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    作者:Yan Sun, Liang Zhu, Pian Liu

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    影響因子 :11.200

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    影響因子 :10.200

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    DOI:10.1016/j.scitotenv.2023.166057

    影響因子 :9.800

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    DOI:10.1016/j.biopha.2023.114419

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    DOI:10.1515/nanoph-2020-0150

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    作者:Liangliang Zhang, Dagang Zhu, Jiwen Jiang

    期刊:CARCINOGENESIS

    DOI:10.1093/carcin/bgad040

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  • Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes(2023/08/18)

    作者:Chang Gao, Jiafeng Fu, Junyi Cui

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    DOI:10.3390/molecules28166128

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  • MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression(2020/12/18)

    作者:Haiming Ma, Tao Liu, Yanhua Xu

    期刊:OncoTargets and Therapy

    DOI:10.2147/OTT.S281962

    影響因子 :4.345

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    作者:Jinlu An, Jiang Shi, Chao Yang

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    DOI:10.1007/s11010-023-04837-6

    影響因子 :4.300

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    作者:Hongjian Chen, Jie Fan, Xiuxiu Sun

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    DOI:10.3892/etm.2023.12168

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    DOI:10.1016/j.tice.2023.102164

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    DOI:10.3892/mco.2023.2617

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    期刊:Digital Chinese Medicine

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